Figure 5.

VPS13C knock down decreases LD size and triglyceride content. Characterization of control and cells silenced for VPS13C: A) Representative Western blot for VPS13C at different differentiation stages (ND = non-differentiated, 1d-6d = 1–6 days after induction). B) Cumulative data at day 4 after differentiation (n = 3). C) Immunostaining for VPS13C at day 4 after differentiation, green: VPS13C, red: lipids stained with lipidTOX deep red. D) LD size distribution as percentage of total, quantified using confocal images in cells stained with LipidTOX and DAPI (n = 3). Insert shows magnified areas of the graph. E) Triglyceride (TG) content (n = 4).