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. 2017 Nov 12;7:57–70. doi: 10.1016/j.molmet.2017.10.014

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© 2017 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

VPS13C knock down increases targeting of ATGL to LDs. A) Western blot for ATGL in BA cell fractions. Left panel: representative Western blot; right panel: cumulative data (n = 4). KD = BA knocked down for VPS13C; PN = post-nuclear; cyto = cytoplasm; LD = lipid droplets. B) Western blot for phospho-HSL and HSL in total lysates from cells treated with control shRNA (ctrl) or shRNA against VPS13C (KD). Left panel: representative Western blot; right panel cumulative data (n = 4) C) Immunostaining for ATGL in control and VPS13C-silenced BAs.