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. Author manuscript; available in PMC: 2018 Jan 25.
Published in final edited form as: ACS Catal. 2017 Apr 20;7(6):3837–3849. doi: 10.1021/acscatal.7b00408

Figure 2. Overview of Predict-Design-Revise-Learn approach used to guide ‘TesA redesign.

Figure 2

Pictorial illustration of the steps traversed (left column) and mutants identified with improved specificity towards C12- or C8- acyl-ACPs. The right column denotes the changes in the computational procedure modifications in response to the experimental results. Variants that produced significant improvements in the C12 (p<0.05) or C8 (p<0.005) fraction while maintaining WT production levels are indicated with an upward arrow followed by the FFA. Major improvements in C8 are indicated by p<0.005 rather than p<0.05 to highlight top designs Struck through variants indicate enzyme inactivity, and names followed by a downward arrow represent variants with reduced total production levels relative to WT.