Figure 7.
Immunolocalization of MCP-1 and its protein level in T10 DRG at two weeks after STZ administration. Representative images of immunostaining with anti-MCP-1 antibody in control (CT) (a) and diabetic (DM) (b) rats. Scale bar: 50 µm. MCP-1 was constitutively expressed in DRG neurons in both CT and DM rats (n = 4 rats per group). (c) Representative Western blot analyses using anti-MCP-1 and GAPDH antibodies are shown. GAPDH was used as a loading control. CT 1–3: CT rats; DM 1–3: DM rats. (d) Semi-quantitative densitometric data for MCP-1-IR is shown. Data are shown as mean ± SEM, n = 3 rats per group. There was no difference in MCP-1 protein levels between the CT and DM groups.