Table 8.
Combination index values for cell lines tested with both copanlisib and afatinib. ED25, ED50, ED75 values are represented for each cell line. Standard deviations are representative of triplicate independent experiments.
| Cell line | Afatinib (nM) | Copanlisib (nM) | CI at ED75 |
|---|---|---|---|
| Group 1: ERBB family WT/PIK3CA WT | |||
| KLE | 13.5 ± 9.3 | 58.2 ± 13.1 | 3.21 ± 3.05 |
| SW620 | 1353.6 ± 49.4 | 124.4 ± 21.2 | 0.69 ± 0.01 |
| CL-14 | 19.94 ± 8.89 | 132.4 ± 16.7 | 6.54 ± 5.84 |
| Group 2: ERBB family WT/PIK3CA Mut | |||
| HT29 | 3110.0 ± 784.3 | 48.0 ± 6.7 | 1.12 ± 0.27 |
| T47D | 879.7 ± 167.3 | 1.36 ± 0.4 | 1.15 ± 0.48 |
| MFE-280 | 1.38 ± 2.36 | 7.2 ± 1.78 | >50 |
| Group 3: ERBB family Mut/PIK3CA WT | |||
| C2BBe1 | 793.6 ± 90.7 | 107.5 ± 35.0 | 0.36 ± 0.11 |
| LoVo | 12.0 ± 3.6 | 57.0 ± 4.3 | 0.68 ± 0.26 |
| CaOV3 | 21.3 ± 13.0 | 212.4 ± 56.0 | 6.72 ± 1.02 |
| Group 4: ERBB family Mut/PIK3CA Mut | |||
| H1975 | 113.2 ± 55.1 | 12.81 ± 0.63 | 0.59 ± 0.19 |
| MCF-7 | 2286 ± 238 | 4.52 ± 1.40 | 0.57 ± 0.07 |
| HCT116 | 1005.8 ± 175.2 | 28.03 ± 2.96 | 0.59 ± 0.14 |
CI, combination index; ED, effective dose; Mut, mutation; WT, wild type.