Table 9.
The full list of antibodies used in the RPPA assay.
| Antibody | Cat No. | Company | Dil | Host | |
|---|---|---|---|---|---|
| 1 | AKT | 4691 | CellSig Massachusetts, USA | 1:3000 | R |
| 2 | AKT (S473) | 9271 | CellSig | 1:250 | R |
| 3 | AKT (T308) | 2965 | CellSig | 1:500 | R |
| 4 | AMPK (T172) | 2535 | CellSig | 1:250 | R |
| 5 | AMPKalpha1 | 2532 | CellSig | 1:250 | R |
| 6 | C-Raf | 04-739 | Millipore Massachusetts, USA | 1:250 | R |
| 7 | c-Raf (S338) | 9427 | CellSig | 1:200 | R |
| 8 | EGFR | 2232 | CellSig | 1:100 | R |
| 9 | EGFR (Y1173) | 1124 | Epitomics California, USA | 1:50 | R |
| 10 | EGFR (Y992) | 2235 | CellSig | 1:100 | R |
| 11 | EGFR (Y1068) | 2234 | CellSig | 1:100 | R |
| 12 | HER2 | MS-325-P1 | Lab Vision California, USA | 1:1000 | M |
| 13 | HER2 (Y1248) | 06-229 | Upstate Massachusetts, USA | 1:750 | R |
| 14 | HER3 | 285 | Santa Cruz California, USA | 1:500 | R |
| 15 | HER3 (Y1289) | 4791 | CellSig | 1:50 | R |
| 16 | IGFIR-β | 3027 | CellSig | 1:500 | R |
| 17 | IGFIR-β (Y1135/1136) | 3024 | CellSig | 1:500 | R |
| 18 | MAPK-ERK 1/2 | 9102 | CellSig | 1:200 | R |
| 19 | MAPK (T202/Y204) -ERK1/2 | 4377 | CellSig | 1:1200 | R |
| 20 | MEK1 | 1235-1 | Epitomics | 1:1200 | R |
| 21 | MEK1/2 (S217/221) | 9154 | CellSig | 1:1000 | R |
| 22 | mTOR | 2983 | CellSig | 1:400 | R |
| 23 | mTOR (S2448) | 2971 | CellSig | 1:100 | R |
| 24 | p38 MAPK (T180/Y182) | 9211 | CellSig | 1:250 | R |
| 25 | p38_MAPK | 9212 | CellSig | 1:300 | R |
| 26 | p70 S6 kinase | 1494-1 | Epitomics | 1:250 | R |
| 27 | p70 S6 kinase (T389) | 9205 | CellSig | 1:250 | R |
| 28 | S6 ribosomal protein (S235/236) (2F9) | 4856 | CellSig | 1:200 | R |
| 29 | S6 ribosomal protein (S240/244) | 2215 | CellSig | 1:3000 | R |
AKT, protein kinase B; AMPK, 5’ AMP-activated protein kinase; c-RAF, c-Raf proto oncogene serine/threonine protein kinase; EGFR, epidermal growth factor receptor; HER2, human epidermal growth factor receptor 2; HER3, human epidermal growth factor receptor 3; IGFIR-B, insulin-like growth factor I receptor β; MAPK, mitogen-activated protein kinase; MEK1, mitogen-activated protein kinase kinase; mTOR, mammalian target of rapamycin; p70 S6 kinase, ribosomal protein S6 kinase beta-1.
CellSig, Cell Signalling Technology Netherlands; Millipore, Merck Millipore USA; Lab Vision, Thermo Fisher Scientific USA; Upstate, Merck Millipore USA; Santa Cruz, Santa Cruz Biotechnology USA; Epitomics, An Abcam Company USA.