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. 2018 Jan 17;17:1533034617749418. doi: 10.1177/1533034617749418

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© The Author(s) 2018

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 4. — Lapatinib inhibits breast cancer cell proliferation by influencing PKM2 expression. A, Lapatinib inhibits MDA-MB-231 cell proliferation. MDA-MB-231 cells were treated with lapatinib and DMSO. The results showed that cells proliferated at a significantly slower rate in the lapatinib treatment group compared with DMSO treatment and untreated groups (P < .05). There were no obvious changes in the DMSO treatment group compared to the untreated group in terms of cell proliferation. Both DMSO treatment groups and untreated groups served as control groups. Data represent the means (SD) of 6 independent experiments. B, Lapatinib inhibits SK-BR-3 cell proliferation. SK-BR-3 cells were treated with lapatinib and DMSO. The results showed that cells proliferated at a significantly slower rate in the lapatinib treatment group compared to DMSO treatment and untreated groups (P < .05). There were no obvious changes in cell proliferation in the DMSO treatment group relative to the untreated group. Dimethyl sulfoxide treatment groups and untreated groups served as control groups. Data represent the means (SD) of 6 independent experiments. C and E, The pcDNA3.1-PKM2 recombinant plasmid was successfully transfected into MDA-MB-231 and SK-BR-3 cell lines. WT represents the wild-type group. Vec represents the negative control group. The experiments were repeated 3 times. D and F, Lapatinib inhibits breast cancer cell proliferation by influencing PKM2 expression. The PKM2 sequences were transfected into MDA-MB-231 and SK-BR-3 cell lines under treatment with lapatinib for overexpression. The CCK-8 results show that cells proliferated at a significantly faster rate in transfected groups compared to control groups (P < .05), and there was no obvious change between negative control groups and wild-type groups. WT represents the wild-type group. Vec represents the negative control group. Data represent the means (SD) of 6 independent experiments. CCK-8 denotes Cell Counting Kit-8; DMSO, dimethyl sulfoxide; PKM2, pyruvate kinase type M2; SD, standard deviation; MDA-MB-231, Human breast cancer cells MDA-MB-231; SK-BR-3, Human breast cancer cells SK-BR-3.