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. 2018 Jan 22;13:479–492. doi: 10.2147/IJN.S154968

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© 2018 Zhang et al. This work is published and licensed by Dove Medical Press Limited

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CLSM of RWM as a function of administration time. (A) CLSM micrographs of outer epithelium cells of RWM after intratympanic injection of 0.1 mL of 90 mg/mL PLGA NPs for 10, 30, and 60 minutes. DAPI-stained cell nuclei are blue and PLGA NPs are green. (B) Coumarin-6 fluorescence intensity of RWM at different distances along the z-axis from middle ear side to inner ear side. Three-dimensional reconstruction images of RWM obtained from successive z-stacks after intratympanic injection of NPs for 10 minutes (C), 30 minutes (D), and 60 minutes (E).

Abbreviations: CLSM, confocal laser scanning microscope; RWM, round window membrane; DAPI, 4′,6-diamidino-2-phenylindole; PLGA NPs, poly (lactic-co-glycolic acid) nanoparticles.