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. 2018 Jan 22;11:473–482. doi: 10.2147/OTT.S143440

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© 2018 Xie et al. This work is published and licensed by Dove Medical Press Limited

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The expression of HOXC10 was decreased after RNA interference.

Notes: (A and B) mRNA and protein expression of HOXC10 in five OS cell lines (n=3). HOXC10 was overexpressed in SaoS2 and MG63 cell lines. These two cell lines were selected to perform further assays. (C) mRNA of HOXC10 was remarkably silenced after siRNA transfected in three OS cell lines (n=3). (D and E) The protein level of HOXC10 was obviously decreased after RNA interference in three OS cell lines (n=3). Data shown as mean ± SD, **P<0.01, ***P<0.001 (compared with negative controls).

Abbreviations: HOXC10, Homeobox C10; NC, nonspecific control siRNA; OS, osteosarcoma.