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. 2018 Jan 24;49:8. doi: 10.1186/s13567-018-0504-3

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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PPRV replication and nectin-4 expression in EECs. A Morphological changes in infected EECs at the indicated time points (magnification, ×100). B PPRV-infected cells were collected for western blotting with anti-PPRV-N and anti-nectin-4 antibodies at the indicated time points. β-Actin was detected as the loading control. Representative results are shown and similar results were obtained in three independent experiments. C Analysis of the relative levels of nectin-4 and PPRV-N in infected EECs. The optical densities for the nectin-4, PPRV-N, and β-actin protein bands were measured with densitometric scanning, and the ratios of nectin-4/β-actin and PPRV-N/β-actin were calculated. The data are expressed as the mean ± SD of three independent experiments.