Table 1.
Summary of advantages/disadvantages of immune-deficient mouse strains for PDX
| Mouse strain | Deficiency | Median survival | Advantage | Disadvantage |
|---|---|---|---|---|
| Nude | No functional T cell | Not determined | Well characterized | Functional B and NK cell |
| High take-rate of human tumor | T-cell functionality increases with age | |||
| Not suitable for primary cell transplantation | ||||
| SCID | No functional T and B cell | Not determined | Better engrafment of allogeneic and xenogeneic tumor cells and tissues than in nude strain | Functional NK cells |
| Spontaneous lymphomas | ||||
| NOD-SCID | No functional T and B cell | 36 wk | Low NK cell activity | High incidence of lymphomas |
| NK cell impaired | Very low leakiness with age | Radiosensitive | ||
| Engrafts hematopoietic cancer cell line | ||||
| NSG | No functional T, B, and NK cell | > 89 wk | Lymphoma-resistant excellent engraftment of allogeneic and xenogeneic tumor cells and tissue | Not well charaterized |
| Suitable for analysis of human cancer stem cells and metastasis |
PDX, patient-derived xenograft; NK, natural killer; SCID, severely compromised immune deficient; NOD, nonobese diabetic; NSG, NOD-SCID mice with IL-2Rγnull.