Table 1.
S. cerevisiae strains used in this study
| Strain name | Relevant genotype | Parental strain | Origin |
|---|---|---|---|
| CEN.PK113-5D | MATa ura3-52 | – | [29] |
| CEN.PK122 | MATa/MATα | – | [29] |
| IMX585 | MATa URA3 can1::cas9-natNT2 | CEN.PK113-7D | [34] |
| IMX581 | MATa ura3-52 can1::cas9-natNT2 | CEN.PK113-5D | [34] |
| IMX673 | MATa/MATα ura3-52/ura3-52 CAN1/can1Δ::cas9-natNT2 | CEN.PK115 | [34] |
| IME324 | MATa ura3-52 can1::cas9-natNT2 p426-TEF (empty) | IMX581 | [22] |
| IMX765a | MATa/MATa ura3-52/ura3-52 can1::cas9-natNT2/can1::cas9-natNT2 sga1::cbbm (9 copies), groES, groEL/sga1::cbbm (9 copies), groES, groEL | IMX581 | This study |
| IMX773 | MATa/MATa ura3-52/ura3-52 can1::cas9-natNT2/can1::cas9-natNT2 sga1::cbbm (9 copies), groES, groEL/sga1::cbbm (9 copies), groES, groEL X-2::pYEN1-prk/X-2::pYEN1-prk pUDR164 | IMX765 | This study |
| IMX774 | MATa/MATa ura3-52/ura3-52 can1::cas9-natNT2/can1::cas9-natNT2 sga1::cbbm (9 copies), groES, groEL/sga1::cbbm (9 copies), groES, groEL X-2::pDAN1-prk/X-2::pDAN1-prk pUDR164 | IMX765 | This study |
| IMX949 | MATa/MATa ura3-52/ura3-52 can1::cas9-natNT2/can1::cas9-natNT2 sga1::cbbm (9 copies), groES, groEL/sga1::cbbm (9 copies), groES, groEL X-2::pDAN1-prk/X-2::pDAN1-prk gpd2Δ/gpd2Δ pROS10-gRNA.GPD2 | IMX774 | This study |
| IMX1443 | MATa/MATa ura3-52/ura3-52 can1::cas9-natNT2/can1::cas9-natNT2 sga1::cbbm (9 copies), groES, groEL/sga1::cbbm (9 copies), groES, groEL X-2::pDAN1-prk/X-2::pDAN1-prk gpd2::pTDH3-RPE1, pPGK1-TKL1, pTEF1-TAL1, pPGI1-NQM1, pTPI1-RKI1, pPYK1-TKL2/gpd2::pTDH3-RPE1, pPGK1-TKL1, pTEF1-TAL1, pPGI1-NQM1, pTPI1-RKI1, pPYK1-TKL2 pUDR164 | IMX774 | This study |
| IME369 | MATa/MATα ura3-52/ura3-52 CAN1/can1Δ::cas9-natNT2 p426-TEF (empty) | IMX673 | This study |
| IMX1472 | MATa ura3-52 can1::cas9-natNT2 gpd2::pTDH3-RPE1, pPGK1-TKL1, pTEF1-TAL1, pPGI1-NQM1, pTPI1-RKI1, pPYK1-TKL2 pROS11-gRNA.GPD2 | IMX581 | This study |
| IMX1489 | MATa ura3-52 can1::cas9-natNT2 gpd2::pTDH3-RPE1, pPGK1-TKL1, pTEF1-TAL1, pPGI1-NQM1, pTPI1-RKI1, pPYK1-TKL2 sga1::pDAN1-prk, cbbm (9 copies), groES, groEL pUDR103 | IMX1472 | This study |
aIndicates spontaneous diploidization; see “Results” section