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. 2018 Jan 15;35(2):341–352. doi: 10.1089/neu.2017.5155

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© Eric Peter Thelin et al., 2017. Published by Mary Ann Liebert, Inc.

This Open Access article is distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited.

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FIG. 2. — (A) Phase contrast image ( × 200) showing the enriched neuronal cultures that were used in the experiments. (B) Illustrates immunocytochemistry of the neuronal culture with βIII-tubulin stained neurons (green) and DAPI (blue) as cellular counterstaining (scale bar: 50 μm). (C) Confirms neuronal and neurotransmitter markers by staining for synapsin, glutamate, MAP2ab, and GABA (red), DAPI (blue), and βIII-tubulin (green) (scale bar: 50 μm). (D) Illustrates cortical markers, resulting as a default regional identity after neural induction by staining for FOXG1, OTX1, TBR1, and REELIN (scale bar: 25μm). βIII-tubulin, Beta-III-tubulin; DAPI, 4',6-diamidino-2-phenylindole; MAP2ab, microtubule associated protein 2ab; GABA, gamma-aminobutyric acid; FOXG1, Forkhead Box G1; OTX1, orthodenticle homeobox 1; TBR, T-Box, Brain 1.