Figure 2. Activation of P2YR (P2Y₁₁ and P2Y₁₃) is responsible for the amplitude of ATP-evoked Ca²⁺ response in GM-MDM cells.

A) ATP concentration response (0.01 μM – 300 μM) in presence and absence of extracellular Ca²⁺ in GM-MDM cells (N=3 donors). B) Representative time response traces of Ca²⁺ response in response to 100 μM ATP in the presence and absence of extracellular Ca²⁺ (N=3 donors). Effect of P2Y receptor selective antagonists on ATP-evoked Ca²⁺ response: C) P2Y₁₁ (10 μM NF340; N=3 donors) and D) P2Y₁₃ (10 μM MRS2211; N=3 donors), E and F) Effect of P2Y₁₁ and P2Y₁₃ selective antagonists on ATP-evoked Ca²⁺ response (N=3 donors). Statistical significance is represented as * p<0.05, *** p<0.001