Figure 3. Activation of P2X₄ contribute to the sustained phase of the ATP-evoked Ca²⁺ response.

A and B) Effect of P2X₄ selective allosteric modulator (3 μM IVM; N=12 donors) represented as time response curves and area under the curve of Ca²⁺ response, respectively. C to F) Effect of P2X₄ selective antagonist, 10 μM PSB-12062 (C and D; N=12 donors) and 5 μM BX430 (E and F; N=5 donors) represented as time response curves and area under the curve of Ca²⁺ response. G) Effect of co-application of selective P2X₄ receptor antagonist and P2Y receptor antagonists (P2Y₁₁ – NF340 and P2Y₁₃ – MRS2211) on peak magnitude of Ca²⁺ response (N=4 donors). Statistical significance is represented as ** p<0.01.