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. Author manuscript; available in PMC: 2018 Aug 1.
Published in final edited form as: J Immunol. 2017 Dec 18;200(3):1159–1168. doi: 10.4049/jimmunol.1700965

Figure 5. P2X4 receptor modulates ATP-induced mRNA expression and protein secretion of CXCL5 in human MDM.

Figure 5

A) qRT-PCR analysis of effect of blocking P2X4 (10 μM PSB-12062; N=6 donors) over different time points (6h, 9h, 18h and 24h). B & C) Effect of PSB-12062 on ATP-induced CXCL5 protein secretion at 24h (N=6 donors), 32h (N=6 donors) and 48h (N=7 donors), as quantified by ELISA. Black line: ATP control, red line: ATP + 10 μM PSB-12062, dark grey line: 10 μM PSB-12062 control, light grey line: vehicle control. D) Effect of concentration-response of PSB-12062 on ATP-induced CXCL5 protein secretion at 48h (N=4 donors). E) Effect of 5 μM BX430 on ATP-induced CXCL5 secretion at 48h (N=7 donors). F) Effect of 3 μM IVM on ATP-induced CXCL5 protein secretion at 48h (N=6 donors). G) Effect of extracellular Ca2+ depletion (2 mM EGTA) on constitutive and ATP-induced CXCL5 secretion (N=7 donors). H) Effect of P2X4 receptor antagonists (10 μM PSB-12062 and 5 μM BX430) on ATP-induced CXCL5 secretion in the presence (N=7 donors) or absence of extracellular Ca2+ (N=4 donors). Statistical significance is represented as * p<0.05, ** p<0.01 and *** p<0.001.