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. Author manuscript; available in PMC: 2019 Jan 29.
Published in final edited form as: Biochem Biophys Res Commun. 2018 Jan 5;496(1):205–211. doi: 10.1016/j.bbrc.2018.01.027

Figure 2. IC50 determinations for Compound 1s in selected kinase activity assays.

Figure 2

IC50 determinations for Compound 1s were performed by Carna Biosciences using the indicated kinase activity assays. Biochemical kinase activity assays were performed using the indicated target kinase and another kinase as substrate in ELISA-based activity assays where the [ATP] was at or near the respective Km values for each kinase assay. Compound 1s was tested in duplicate at each of the 10 indicated concentrations for each assay and the mean ± SD percent activity was plotted. Calculated IC50 values were 60 nM for MEKK2 (●), 53 nM for MEKK3 (■), >1 μM for MAP2K5 (▼) and >10 μM for TAK1-TAB1 (◆) and MAP3K5 (▲).