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. Author manuscript; available in PMC: 2018 May 6.
Published in final edited form as: Nat Chem. 2017 Nov 6;10(2):155–164. doi: 10.1038/nchem.2872

Figure 2. PER mechanism.

Figure 2

(a) A primer exchange reaction, which uses a catalytic hairpin to produce transcripts of the form a–b. (b) An example reaction implementation with a hairpin that uses a G-C pair as a stop sequence. See Supplementary Note 2 for other types of stop sequences that can be used. (c) The primer exchange reaction cycle. (d) PAGE denaturing gel depicting a reaction time series of a primer exchange reaction. Primer and hairpin concentrations were fixed at 100 nM and 1 nM respectively, and reactions were incubated at 37 °C with 10 μM of dATP, dTTP, and dCTP. See Methods section and Supplementary Note 3 for additional details.