Fig 3. Effect of increasing amounts of EP on the cytotoxic effects of Cu²⁺-oxidized LDLs in EA.hy926 cells.

nLDL (1.5 mg/mL) was preincubated in the absence or presence of 500 or 1000 μg/mL EP and then oxidized by addition of 10 μmol/L CuCl₂ till LPO levels reached 100 nmol/mg LDL protein. Subsequently, EA.hy926 cells were incubated with the thus obtained oxLDLs. (A) Cell viability concentration-dependently increased and (B) intracellular superoxide radical anion formation concentration-dependently decreased with oxLDLs obtained under increasing levels of EP. Data represent mean ± SD (n = 7), *** p < 0.001.