Fig 3. Curcumin treatment increases the survival of host cells and suppresses EV71 replication.

(A) Cells were seeded in culture plates and treated with 10 μM curcumin. After one hour, the medium was removed, and the cells were infected with EV71 at the MOI of 1 in curcumin-containing serum-free medium. After adsorption, the medium was removed, and medium containing 2% serum and 10 μM curcumin was added. The growth curves of infected cells were counted using a trypan blue exclusion assay. (B) Cell lysates were collected from cell samples, and a plaque assay was performed to determine the virus titers. (C) Cells were harvested at different time points, and total protein was extracted to determine the expression of EV71 3D protein by Western blot. The expression of β-actin was used as an internal control. (D) RT-qPCR analysis was performed to detect the amounts of viral RNA.