Fig 5. Curcumin does not suppress EV71 by modifying MAPK pathways.

(A) Cells were infected with EV71 at different MOIs. Total cell lysates were harvested at different time points, and Erk and p38 phosphorylation was detected by immunoblot analysis. (B) To detect the effect of curcumin on MAPK pathways, HT29 cells were seeded in plates and infected with EV71 at the MOI of 1 with or without 10 μM curcumin in the medium. The cells were harvested at different time points, and total protein was isolated for Western blot analysis. Anti-P-Erk, anti-P-p38, anti-Total Erk, anti-Total p38, and anti-EV71 3D antibodies were applied to detect the phosphorylation of kinases.