Embryos at stage 32 are shown in lateral views (A,E) and transversal sections (B-D’, F-H’). (A)
Dse expression. The bold lines indicate the level of sections in B-D. (B-D’)
Dse expression is found in the cranial neural crest (indented arrowheads). Note ventral expression domains in the forebrain, posterior midbrain, hindbrain, and notochord. Weak expression is visible in the trigeminal ganglia. (E)
Dsel expression. The bold lines indicate the level of sections in F-H. (F-H’)
Dsel expression appears in the cranial neural crest (indented arrowheads) and trigeminal ganglia. Note expression domain in the prechordal mesendoderm. Apparent are low-level transcripts in the entire ventral brain and robust bilateral expression domains in the ventral marginal zone of the mid- and hindbrain (arrows). fb, forebrain; hb, hindbrain; he, heart; mb, midbrain; no, notochord; pm, prechordal mesendoderm; V, trigeminal ganglion. Scale bars are 1 mm (A-H) and 200 μm (B’-H’).