Fig 6. Relative expression of MtDefMd1 and selected AM marker genes in mycorrhized MtDefMd1-overexpression and pPT4:gusAint-expressing transgenic control roots of M. truncatula.

Transcript amounts are shown relative to MtTefα (A) and were additionally normalized by building a ratio to MtPt4-expression (B). Measurements of pPT4:MtDefMd1 overexpression roots are coloured in light grey, measurements of pUbi:MtDefMd1 overexpression roots in medium grey, and measurements of pPT4:gusAint control roots in dark grey. Roots were harvested at 28 days post inoculation with R. irregularis. n = 12 biological replicates, depicted is the standard error of the mean. Statistical significance: * p≤0.05.