Fig 7. Size distribution of arbuscules in mycorrhized M. truncatula MtDefMd1-overexpression, MtDefMd1/2-knock-down, and pPT4:gusAint controls roots.

Arbuscules were sorted into one of eleven size categories. In total, the size distribution of arbuscules in pUbi:MtDefMd1-overexpression (647 arbuscules), pPt4:MtDefMd1-overexpression (509 arbuscules), RNAi:MtDefMd1/2-knock-down (625 arbuscules), and pPt4:gusAint control roots (529 arbuscules) is depicted in orange, blue, green, and grey, respectively. Roots were harvested at 28 days post inoculation with R. irregularis. For each construct, three pools of root fragments, each pool being derived from four plants, were analysed. Depicted is the standard error of the mean.