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. 2018 Jan 25;13(1):e0191886. doi: 10.1371/journal.pone.0191886

Fig 7. Gel electrophoresis of PCR products for the diagnosis of a clinical sample.

Fig 7

(A) The first PCR products were visualized on 2% agarose-TAE gel containing GelRed (Wako). Lane L indicates a molecular marker (100-bp ladder). The letters shown below each lane indicate the template DNA used for the first PCR reactions (lane S indicates a DNA sample from a patient’s blood; lanes F, V, Oc, M, K, and Ow indicate plasmid DNA containing each partial sequence of the SSU rRNA genes from P. falciparum, P. vivax, P. ovale curtisi, P. malariae, P. knowlesi, and P. ovale wallikeri, respectively; lane N, indicates the negative control [water]). The PCR products are shown at 136–159 bp. (B) The second PCR products were visualized on 2% agarose-TAE gel containing GelRed (Wako). Lane L indicates a molecular marker (100-bp ladder). The letters shown below each lane indicate the template used for the second PCR reactions (lane S indicates the diluted first PCR product amplified from a DNA sample from a patient’s blood; lanes F, V, Oc, M, K, and Ow indicate the diluted first PCR products amplified from the sequences of P. falciparum, P. vivax, P. ovale curtisi, P. malariae, P. knowlesi, and P. ovale wallikeri, respectively; lane N indicates the diluted first PCR product from water; lane N’, water). The arrow indicates the PCR product amplified from a DNA sample from the blood of a patient with P. vivax-specific primers. The primer sets are indicated below the gel.