Fig. 3.

Neutralization of HCVcc by mAbs 2A5 and AP33. (A) HCVcc expressing the structural proteins of genotype 1a, 1b, 2a, 3a, 4a, 5a, 6a and 7a isolates were pre-incubated with three-fold serial dilutions of mAbs 2A5, AP33 or a control Ab. The mixture was transferred to Huh7.5.RFP cells and incubated for 4 h before washing. Two days later, HCV-infected foci were visualized using an NS5A-specific antibody and counted. Results are expressed as percentage of infectivity (mean ± standard deviation (error bars)). (B) IC₅₀ values were calculated from dose-response curves shown in (A). All conditions were performed in triplicate.