| i. | 2 min | 94°C | (polymerase activation) |
| ii. | 10 secs | 98°C | (denaturation) |
| iii. | 30 secs | lowest primer Tm°C | (annealing) |
| iv. | 1 min | 68°C | (extension) |
| Repeat steps ii–iv for 40 cycles. | |||
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