1.
Characteristics of the metabolism-related antibodies used for immunohistochemistry.
| Target protein | Ab raised against | Clone No. | Concentration
and time |
Subcellular localization | |
| 1 | Glucose transporter 1 | Rabbit polyclonal to GLUT1 raised against a synthetic peptide of human GLUT1 at N-terminus | Ab15309
ABCAM, UK |
1:100
overnight |
Cell membrane |
| 2 | Glucose transporter 2 | Goat polyclonal to GLUT2 raised against a synthetic peptide corresponding to human GLUT2 | Ab 111117
ABCAM, UK |
1:100
overnight |
Cell membrane |
| 3 | Monocarboxylic acid transporter 1 | Rabbit polyclonal to MCT1 raised against a synthetic peptide of human MCT1 at C-terminus. | Ab85021
ABCAM, UK |
1:150
overnight |
Cell membrane |
| 4 | Monocarboxylic acid transporter 2 | Goat polyclonal to MCT2 raised against a synthetic peptide of human MCT2 at C-terminus. |
Ab129290
ABCAM, UK |
1:50
overnight |
Cell membrane |
| 5 | Hexokinase II | Mouse monoclonal antibody raised against recombinant fragment of hexokinase II |
Ab104836
ABCAM, UK |
1:50
overnight |
Cytoplasmic |
| 6 | Fructose-6-phosphate kinase | Rabbit polyclonal to fructose-6-phosphate kinase liver type (phosphofructokinase) raised against a synthetic peptide of human PFKL | Ab 37583
ABCAM, UK |
1:50
overnight |
Cytoplasmic |
| 7 | Fructose bisphosphate aldolase | Mouse monoclonal to aldolase antibody raised against recombinant full length protein, corresponding to amino acids 1-365 of human aldolase | Ab54770
ABCAM, UK |
1:200
overnight |
Cytoplasmic |
| 8 | Lactate
dehydrogenase V (M4) |
Sheep polyclonal antibody against human LDH-V from human placenta | Ab9002
ABCAM, UK |
1:200
overnight |
Cytoplasmic |
| 9 | Lactate
dehydrogenase-I (H4) |
Rabbit polyclonal antibody to LDH-I (H4) raised against a synthetic peptide of the human LDH isozyme H4 | Ab81485
ABCAM, UK |
1:200
overnight |
Cytoplasmic |
| 10 | Pyruvate dehydrogenase | Mouse monoclonal against porcine pyruvate dehydrogenase E2/Ebp |
Ab110333
ABCAM, UK |
1/100
overnight |
Cytoplasmic |
| 11 | Phosphorylated pyruvate dehydrogenase | Rabbit polyclonal to phosphorylated PDH at S293 E1-alpha subunit | Ab92696
ABCAM, UK |
1/100
overnight |
Cytoplasmic |
| 12 | Pyruvate dehydrogenase kinase 1 | Mouse monoclonal to mitochondrial pyruvate dehydrogenase kinase 1 raised against recombinant fragment of the protein |
Ab110025
ABCAM, UK |
1:500
overnight |
Cytoplasmic |