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. 2017 Nov 3;14(11):1592–1605. doi: 10.1080/15476286.2017.1338241

Figure 5.

Figure 5.

rpfA riboswitch activity during vegetative growth. S. coelicolor wild-type strains, constitutively expressing wild-type or mutant rpfA riboswitches transcriptionally fused to the luciferase reporter gene cluster, were grown on a sporulation-conducive solid medium. Background-subtracted reporter activity was measured after 44 h. Data are expressed as mean ± standard error (n = 3). Group means annotated with different letters are significantly different. P0: F(3,8) = 38.12, p < 0.001; P1: F(3,8) = 29.87, p < 0.001; P2: F(3,8) = 193.49, p < 0.001; P4: t(4) = −5.80, p = 0.004; P6: t(4) = −15.82, p < 0.001; LIN: t(4) = −17.42, p < 0.001; P5–7: t(4) = −10.15, p < 0.001; P3–7: t(4) = −18.01, p < 0.001. Figure is representative of at least 2 independent experimental replicates. a.l.u., arbitrary luminescence units; WT, M145/pMC222; P0Prox., M145/pMC232; P0Dist., M145/pMC233; P0Comp., M145/pMC234; P1Prox., M145/pMC235; P1Dist., M145/pMC236; P1Comp., M145/pMC237; P2, M145/pMC223; P2Prox., M145/pMC238; P2Dist., M145/pMC239; P4, M145/pMC224; P6, M145/pMC225; LIN, M145/pMC231; P5–7, M145/pMC226; P3–7, M145/pMC227.