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. 2017 May 23;14(11):1514–1521. doi: 10.1080/15476286.2017.1317912

Figure 5.

Figure 5.

The pattern of the V3 fragment obtained by aligning all NR fragments from the 5 ‘and 3′ ends, leaving the middle part unaligned because of the occurrence of gaps and the consequent degeneracies. Bases found at a frequency of ≥ 95% are indicated; otherwise, they are replaced with an asterisk. Sequences containing degeneracies (W, V, R, H, T, B, S, D, M, Y, N) indicates the need for 2 or more bases to reach 95% consensus. Position numbers correspond to E coli 16 S rRNA.