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. 2017 Sep 5;14(11):1580–1591. doi: 10.1080/15476286.2017.1338232

Figure 3.

Figure 3.

Editing Assay in HEK293T cells. (A) Scheme of a editing reporter construct (RNAG) constitutively expressing RFP and GFP only after editing of an amber-stop codon to a tryptophan codon (UAG->UGG). (B) FACS analysis of transfected HEK293T. Increase of the green to red fluorescence ratio indicates increase in editing level. All cells are transfected with the reported construct RNAG. White bar: HEK293T cells transfected with RNAG vector alone; Gray bar: HEK293T cells transfected with RNAG and ADAR2 vectors; Dark gray bar: HEK293T cells transfected with RNAG, ADAR2 and FMRP vectors. Data are presented as means ± SEM. Statistical analysis was performed using one-way ANOVA (***p <0,001; $$p < 0,01). NLS: nuclear localization signal.