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. Author manuscript; available in PMC: 2018 Jul 11.
Published in final edited form as: Cell Rep. 2017 Jul 11;20(2):344–355. doi: 10.1016/j.celrep.2017.06.040

Figure 3. Pharmacologically-induced seizures are sufficient to increase ΔFosB and suppress c-Fos in NTG mice within days.

Figure 3

(A,B) At 2 hrs after single administration of 15 or 25 mg/kg KA, there were increases in ΔFosB IR (n = 4/group) and c-Fos IR (n = 4/group). (C) ΔFosB IR is increased 3 days after single administration 15 or 25 mg/kg KA (n = 4/group). (D) c-Fos IR is increased 3 days after single administration of 5 or 10 mg/kg KA, but decreased 3 days after 15 or 25 mg/kg KA (n = 4/group). (E) ΔFosB and c-Fos expression are positively correlated with increasing doses of KA when assessed 2 hrs after KA injection. (F) ΔFosB and c-Fos expression are inversely correlated with increasing doses of KA when assessed 3 days after KA injection. (G) Summary of results from A–D. “0” indicates no change from control, whereas “+” and “−“ indicate increase and decrease from control, respectively. (H) Increased ΔFosB binding to the c-Fos promoter in mice 3 days after 15 mg/kg KA (n = 5/group). (I) Decreased Fos mRNA expression in mice 3 days after 15 mg/kg KA (n = 8–9/group). (J) NTG mice treated with 3 weekly doses of saline, but not 15 mg/kg KA, show preference for the displaced object in the test phase of the object location memory task (n = 6/group). Data were normalized to saline-treated control values (rel to Sal). *p<0.05, **p<0.01, ***p<0.001. ns, non-significant. Error bars indicate SEM. See also Figures S1 and S3 and Tables S3–S4.