Figure 2. BLACAT2 overexpression promotes LN metastasis of bladder cancer cells in vivo.
(A) Representative images of enucleated popliteal LNs (left panel) inoculated with the indicated cells (n = 12 per group) and histogram analysis of the LN volume (right panel). Scale bar: 5 mm. Error bars indicate SD of the mean. Statistical significance was assessed using 2-tailed Student’s t test. **P < 0.01. (B) Representative images of the popliteal LNs analyzed by H&E staining and IHC staining using an anti-luciferase antibody (n = 12 per group). Scale bars: 500 μm (black); 50 μm (red).