Skip to main content
. 2018 Jan 16;128(2):805–815. doi: 10.1172/JCI96113

Figure 5. Relationship between PD-L1+ APCs and clinical response to PD-1 blockade in patients with metastatic melanoma.

Figure 5

(A–E) Multiplexed immunofluorescence analysis of PD-L1 expression in metastatic melanoma tissues (A–D) and draining lymph nodes (E). (A) Representative image of PD-L1 expression (red) in tumor cells (PAN-melanoma, blue) and nontumor cells in melanoma tissues. (B) PD-L1 expression score was quantified in nontumor cells in patients with complete response (CR), partial response (PR), stable disease (SD), and progressive disease (PD). *P < 0.05. (C) Percentages of PD-L1 expression were quantified in mDCs and macrophages in patients with complete response, partial response, stable disease, and progressive disease. *P < 0.05. (D) Representative images of PD-L1 expression (red) in mDCs (CD11c, green) and macrophages (CD68, blue) in metastatic melanoma tissues. (E) Representative images of PD-L1 expression (red) in APCs (CD11c for DCs, CD163 for macrophages) and tumor cells (Sox10 and Pan-melanoma) in the melanoma-draining lymph nodes. The colocalization of PD-L1+ APCs and PD-1+ T cells is shown.