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. 2018 Jan 16;128(2):826–833. doi: 10.1172/JCI96993

Figure 1. Slc39a8 is expressed in the developing hearts and regulates Zn levels.

Figure 1

(A) qRT-PCR analysis of Slc39a8 in the whole heart at different developmental stages. Gapdh was used as cDNA loading control. n = 3 for each time point. (B) RNA in situ hybridization showed that Slc39a8 is expressed in the trabecular region of E12.5 hearts. Scale bars: 250 μm. (C) qRT-PCR analysis showed that Slc39a8 was efficiently deleted in E12.5 Slc39a8–/– hearts. n = 3 for each genotype. (D) ICP-MS analysis showed that Zn was reduced in E14.5 Slc39a8–/– hearts as compared with Slc39a8+/+ hearts. Results were normalized to protein content. *P = 0.06 by Student’s t test.