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. Author manuscript; available in PMC: 2018 Jun 14.
Published in final edited form as: Cell. 2017 Dec 7;171(7):1573–1588.e28. doi: 10.1016/j.cell.2017.11.008

Figure 3. YY1 Can Enhance DNA Interactions In Vitro.

Figure 3

(A and D) Models depicting the in vitro DNA circularization assays used to detect the ability of YY1 to enhance DNA looping interactions with no motif control (A) or competitor DNA control (D).

(B and E) Results of the in vitro DNA circularization assay visualized by gel electrophoresis with no motif control (B) or competitor DNA control (E). The dominant lower band reflects the starting linear DNA template, while the upper band corresponds to the circularized DNA ligation product.

(C and F) Quantifications of DNA template circularization as a function of incubation time with T4 DNA ligase for no motif control (C) or competitor DNA control (F). Values correspond to the percent of DNA template that is circularized and represents the mean and SD of four experiments.

See also Figure S3.