Skip to main content
. 2017 Aug 5;16(1):292–297. doi: 10.1111/pbi.12771

Figure 3.

Figure 3

Efficiency of the CRISPR‐Cas9‐VQR system is further increased by the UBI1 and ACT1 promoters. (a) The architecture of vectors with different promoters. The promoters of VQR variants are replaced by UBI1 or ACT1. The modified sgRNAs are expressed with U3 promoters. (b) The mutation rate of the CRISPR‐Cas9‐VQR system using different promoters. The efficiency of the CRISPR‐Cas9‐VQR system is greatly increased by using strong promoters and modified sgRNAs. The system with the UBI1 promoter increases the mutation rate by an average of approximately fourfold, whereas that with ACT1 increases the mutation rate by an average of approximately sixfold. (c) The proportion of double and triple mutations using different systems. The proportions of double or triple mutations are dramatically increased. The triple mutations are undetected in the original system.