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. 2017 Jun 20;16(1):176–185. doi: 10.1111/pbi.12758

Figure 5.

Figure 5

Identifying ‘transgene‐clean’ mutant soya bean lines of GmFT2a. (a) Gel image of PCR products obtained with primer sets for two T‐DNA regions of sgRNA/Cas9 vectors. Cas9 (910 bp), part of the Cas9 coding sequence. sgRNA (593 bp), region from the AtU6 promoter to the downstream vector sequence spanning the sgRNA. GmActin was used as a normalization control. M, DL2000 ladder DNA marker. N, negative control (water as template). V, plasmid of the vector used in transformation as template. WT, DNA of wild‐type soya bean plant as template. Labels above the gel: 1–14, individual mutant lines. (b) Detection of the selectable marker gene bar by test strip. WT, wild‐type soya bean plants. Labels 1–14, individual mutant lines. The bands at red arrowhead indicate that bar is positive.