Table 1.
CRISPR/Cas9‐mediated targeted mutagenesis of GmFT2a in the T1 generation
| T1 lines with ft2a mutations | No. of plants sequenced | No. of homozygous ft2a mutants | No. of heterozygous ft2a mutants | No. of plants with no mutation |
|---|---|---|---|---|
| ft2a‐SP1‐T1#5 | 17 | 0 | 11 | 6 |
| ft2a‐SP1‐T1#10 | 14 | 2 | 10 | 2 |
| ft2a‐SP1‐T1#11 | 53 | 16 | 25 | 12 |
| ft2a‐SP1‐T1#16 | 24 | 0 | 18 | 6 |
| ft2a‐SP1‐T1#23 | 8 | 0 | 8 | 0 |
| ft2a‐SP2‐T1#8 | 12 | 8 | 2 | 2 |
| ft2a‐SP2‐T1#10 | 11 | 1 | 8 | 2 |
| ft2a‐SP2‐T1#13 | 13 | 0 | 10 | 3 |
| ft2a‐SP2‐T1#14 | 5 | 0 | 1 | 4 |
| ft2a‐SP2‐T1#15 | 5 | 3 | 0 | 2 |
| ft2a‐SP2‐T1#16 | 12 | 12 | 0 | 0 |
| ft2a‐SP2‐T1#22 | 36 | 10 | 20 | 6 |
| ft2a‐SP2‐T1#36 | 27 | 1 | 9 | 17 |
| ft2a‐SP3‐T1#2 | 8 | 0 | 2 | 6 |
| ft2a‐SP3‐T1#4 | 6 | 0 | 3 | 3 |
| ft2a‐SP3‐T1#10 | 3 | 0 | 2 | 1 |
| ft2a‐SP3‐T1#12 | 8 | 0 | 5 | 3 |
| ft2a‐SP3‐T1#30 | 29 | 11 | 18 | 0 |