Figure 5.

DsRed2 mutation is genetically inheritable from T0 to T1 generation. Seeds (a) and seedlings (b) of YZ1, the control and three representative T1 plants. (c) PCR analysis of Cas9 and DsRed2 in YZ1, CK, the T0 mutant mR1 and T1 progenies. (d) Genotyping of the target DsRed2 gene of independent T0 plants and their T1 progenies. Results for the T0 plants are highlighted in blue textbox. Mutated sites are highlighted in red with grey background. Bars in (a) and (b) are 2 and 3 cm, respectively.