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. 2017 Sep 21;16(1):310–321. doi: 10.1111/pbi.12773

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© 2017 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Subcellular localization of HtNHX1 and HtNHX2 in rice protoplast. Expression of HtNHX1, HtNHX2, HtNHX1del fused with GFP, and AtTPK1 (a), AtHDEL (b)?AtNHX5, AtSYP61, AtScamp (c) fused with RFP in rice protoplast cells.. HtNHX1del: expression of mutated HtNHX1 by deletion of conserved eight amino acids in NHX1 group from different plant species (see Figure S1). AtTPK1, AtHDEL, AtNHX5, AtSYP61 and AtScamp are the localization markers on tonoplast, endoplasmic reticulum, and endosome or trans‐Golgi network/Golgi, respectively, in Arabidopsis.