Fig. 3.
Multiplex PCR performed on tdh (296 bp) and tlh (450 bp) genes on DNA extracted from clams artificially inoculated with increasing concentrations of V. parahaemolyticus ATCC 43996 tenfold serial dilutions (M: 100 bp ladder, Catalogue number G2101, Promega; lane 1: 100 CFU/ml of tenfold dilution; lane 2: 101 CFU/ml; lane 3: 102 CFU/ml; lane 4: 103 CFU/ml; lane 5: 104 CFU/ml; lane 6: 105 CFU/ml; lane 7: 106 CFU/ml; lane 8: positive control; lane 9: negative control; line 10: non-template control) after 0 h (a), 3 h (b) and 6 h (c) of pre-enrichment at 37 °C