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. Author manuscript; available in PMC: 2019 Feb 1.
Published in final edited form as: Dev Biol. 2017 Dec 5;434(1):121–132. doi: 10.1016/j.ydbio.2017.11.018

Figure 1. Respiratory competence of Xenopus endoderm is restricted by the early gastrula stage.

Figure 1

(A) Experimental diagram of the Xenopus respiratory competence assay. Bisected early gastrula stage (NF10.25) Xenopus embryos assayed by in-situ hybridization show the expression of sox17α throughout the definitive endoderm (DE), hhex in anterior endoderm (AE) and ventx2 in posterior endoderm (PE). DE, AE, or PE explants were dissected at NF10.25, cultured in isolation until NF14, treated +/− 50nM RA from NF14–25, and then in 3.5μM Bio + 50ng/mL BMP4 from NF25–38, at which time they were fixed and analyzed by in-situ hybridization for the respiratory endoderm markers nkx2-1 (B–G) and sftpc (H–M).