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. 2018 Jan 25;8:1603. doi: 10.1038/s41598-017-16703-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Drug delivery of ND-EFV through BBB in vitro. ND-EFV (green) and FD (blue) (40 µg/ml) were introduced separately in the upper chamber of in vitro BBB model. EFV drug release was observed at different time points (30 min – Day 2) at the lower chamber of BBB model. A comparative analysis of sustained drug release from ND-EFV vs. FD through BBB was monitored with a drug content of the media with respect to time. Each set of drug release study was done in three replicates and data were represented with the statistical significance (p > 0.0001).