Fig. 1.

CD90/Thy1 expression in small clusters of cells in the dental mesenchyme. a Immuno-fluorescent staining shows CD90/Thy1 expression in the dental mesenchyme between the labial and lingual aspects of the cervical loop at the apical end of the mouse incisor on the sagittal section images. b, c High magnification images show CD90/Thy1 is expressed in small clusters of cells. d Long-chase EdU retaining cells occupy a similar location between the two aspects of epithelial cervical loop. Mice were given EdU for four weeks and chased for another four weeks prior to tissue collection. e, f Co-staining of CD90/Thy1 and EdU demonstrates partially co-localization. g FACS analysis of CD90/Thy1 expression in the postnatal (PN5) mouse dental pulp tissue showing about 12% of pulp cells express CD90/Thy1. h, i Long-chase EdU retention assay shows 4.3% of slow cycling cells are present in the incisor pulp (e), and about 30% of these slow cycling cells express CD90/Thy1