Figure 1.

Anti-PbPfs25DR3 immunization regimes. Groups of 5 mice received each vaccine regime. For each individual experimental regime, the corresponding (negative) control regime is to its immediate right. In each regime, for DFA 5 mice were challenged with P. berghei PbPfs25DR3 to assess for transmission blockade. In regimes 1–6 mice were immunized to attempt to induce a Pfs25 response (‘experimental regimes’). In regimes 7–12 mice were immunized with carrier protein or empty vector controls (‘control regimes’). Regimes 1,2,7,8 use Matrix M as adjuvant, 3,4,9,10 use alhydrogel, 5,6,11,12 are prime/boost regimes, with ChAd63 prime and ookinete boost. All immunizations were performed i.m.