Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Jan 25;8:1570. doi: 10.1038/s41598-018-19817-w

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

ACHE, BCHE expression and enzymatic activity profiling in selected liver cancer cell lines. (a) Human ACHE transcript variants ACHE4-6, ACHE4-5 expression and another set of primers targeting ACHE exons no. 3 and 4 (ACHEex3-4) present in both variants were used for measuring relative ACHE expression by qPCR in the cell lines. Additionally, BCHE expression was measured by qPCR. Relative quantification was performed with respect to the cell lines with the lowest expression and log fold change represented. TPT1 gene was taken as reference and ∆∆Ct method formula was used. (b) AChE enzymatic activity in HCC cell lines was measured using an Ellman method based commercial kit. (c) Mean ACHE/BCHE expression values and enzymatic activities were plotted for observation of correlation. Enzymatic activity numerical values were normalized by division to the highest value. All error bars represent the standard deviation.