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. 2018 Jan 25;8:1570. doi: 10.1038/s41598-018-19817-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The comparison of the AluYb8 Ct values between genotypes using a qPCR assay. DNA was extracted from 3 dpi larvae injected with liver cancer cell lines (Hep3B and SKHep1) were amplified by qPCRs with using ache N, ache S, and AluYb8 primers (Supplementary Table 1). The Ct value difference (∆Ct) between ache N and ache S primers were used for the genotyping (Supplementary Figure 8). (a) For Hep3B (n = 18, 28 and 10 for ache+/+, ache+/− and ache−/−) normalized Ct values were significantly higher in ache−/− larvae in comparison to both ache+/− and ache+/+ larvae (P = 0.005 and 0.0001, respectively). (b) For SKHep1 (n = 13, 26 and 16 for ache+/+, ache+/− and ache−/−) normalized Ct values were significantly higher in ache−/− larvae in comparison to both ache+/− and ache+/+ (P = 0.004 and 0.017, respectively).