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. 2018 Jan 25;8:1617. doi: 10.1038/s41598-018-20005-z

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Neurophysiological recordings from the CNS of third instar larvae of D. melanogaster after CNS specific knockdown of irk2. Representative nerve discharge traces of knockdown control (GFP) flies (A) and the irk2 knockdown strain (B). Initial spike discharge frequencies in spikes/second (Hz) for each experiment are given to the left of each trace. (C) Average baseline spike discharge frequency (Hz) of the wildtype (OR) flies, GFP-knockdown (control), and irk2 knockdown flies. Bars represent mean (n = 25) spike discharge frequency and error bars represent SEM. *Denotes statistical significance at P < 0.01 as determined by a multiple comparisons test.