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. 2018 Jan 25;8:1572. doi: 10.1038/s41598-018-19714-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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NanoBRET association kinetic studies with mepyramine and VUF13816 based fluorescent ligands. Nluc-H₁R HEK293 cells were treated with the indicated concentrations of 10 (a), 11 (b), 12 (c), 23 (d), 24 (e) and 25 (f) and BRET monitored at room temperature every min for 60 min (a–c) or 90 min (d–f). The data shown are representative examples from four (10, 23, 24, 25), five (11) or six (12) independent experiments performed in triplicate.